Xylocarpus Moluccensis Induces Cytotoxicity in Human Hepatocellular Carcinoma HepG2 Cell Line via Activation of the Extrinsic Pathway

Objective: Liver cancer is one of the most common causes death, with reduced survival rates. The development new chemotherapeutic agents essential to find effective cytotoxic drugs that give minimum side effects surrounding healthy tissues. main objective present study was evaluate and mechanism cell death induced by crude diethyl ether extract Xylocarpus mouccensis on human hepatocellular carcinoma line. Methods: cytotoxicity activity measured using MTS assay. mode determined apoptosis study, DNA fragmentation analysis done TUNEL system. pathway or observed caspases 8, 9, 3/7 Glo-caspases method. Results: In this methanol extracts prepared from leaf produced effect IC50 (72hr) < 30µg/ml. value at 72 hours exerted moluccensis 0.22 µg/ml, which more than extract. results obtained colorimetric system suggest (leaf), (leaf) granatum (bark) in HepG2 Besides, caspase-Glo assay demonstrated triggered apoptotic via activation -8, -3/7 However, no visible noticed for caspase -9. Furthermore, TLC indicates presence potential metabolites an moluccensis. Conclusion: Thus, suggests remarkable active as a future therapeutic agent treatment cancer.